Fig. 7.

The effect of HDAC inhibitors on NF-κB target gene activation by TNFα in GT1-7 cells. Activation of NF-κB by TNFα (1 ng/mL; A) and LPS (1 μg/mL; B) was compared by western blotting of phosphorylated NF-κB, after 30 min, 1 h and 3 h treatment with the ratio between phosphorylated NF-κB and total NF-κB graphed normalized relative to controls. TNFα showed the greater induction and so the influence of HDAC inhibitors was tested on TNFα induced gene expression. The effect of HDAC4/5 inhibition by LMK235 and HDAC6 inhibition by Tubastatin A was investigated on TNFα-induced expression of (C) Fos (D) Prkca (E), Prkcd and (F) Ptp1b. qPCR quantification of gene expression is shown relative to Actb levels. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001.