Figure 8.

In vivo toxicity assay in rats.

Notes: Healthy adult Sprague Dawley rats were randomly assigned to four groups, and each group consisted of five rats, which have fasted overnight before experiments. The four groups are M-Gd-NL, Gd-NL, Gd-DTPA, or PBS. All drugs were injected via the tail veins once per day, every other day, for a total of three injections (0.1 mmol/kg Gd-DTPA). Rats were euthanized 24 h after the third injection. The organs of the rats were excised, and hematoxylin and eosin staining was performed on the organs to examine the toxicity of the treatments. Bars represent 100 μm.

Abbreviations: Gd-DTPA, gadolinium-diethylenetriaminepentaacetic; Gd-NL, Gd-DTPA-loaded liposomes; M-Gd-NL, gadolinium-diethylenetriaminepentaacetic-loaded mannosylated liposomes; PBS, phosphate-buffered saline.