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. 2017 Feb 20;12:1415–1430. doi: 10.2147/IJN.S126760

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© 2017 Miao et al. This work is published and licensed by Dove Medical Press Limited

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Behavior of human MG-63 osteoblasts on three types of surfaces.

Notes: (A) Cell morphology after 24 hours of seeding. (B, C) Cell proliferation measured with the WST-8 kit and 4′,6-diamidino-2-phenylindole staining after 1, 3 and 5 days of culture. (D) Osteogenic gene expression (ALP, COL 1 and OCN) measured with quantitative real-time polymerase chain reaction after 7 and 14 days of incubation. (E) ALP activity after culturing for 14 days. (F) Protein expression of OCN detected using immunofluorescence staining after 14 days of culture; OCN (red), actin (green) and nucleus (blue). ***P<0.001 versus Smooth surface.

Abbreviations: ALP, alkaline phosphatase; COL 1, type I collagen; OCN, osteocalcin; OD, optical density.