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. Author manuscript; available in PMC: 2017 Apr 30.
Published in final edited form as: Nat Chem. 2016 Oct 31;9(3):234–243. doi: 10.1038/nchem.2645

Fig. 6. Possible routes for N-terminal processing of SCRN3 in comparison to the established mechanisms of N-terminal maturation for other protein classes.

Fig. 6

The mechanisms for Ntn hydrolases and pyruvoyl-dependent decarboxylases diverge at the transient ester intermediate formed during autoproteolysis (step 1). Hydrolysis yields a catalytic nucleophile for Ntn hydrolases (step 2), whereas active site-directed β-elimination generates an electrophilic pyruvoyl cofactor for decarboxylases (step 3). Detection of a new N-terminus containing an intact side chain, shown as a product of step 2, suggests that formation of pyruvoyl and glyoxylyl groups may occur via 4 and 5, respectively, in the N-terminal processing of SCRN3.