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. 2017 Feb 24;12(2):e0171359. doi: 10.1371/journal.pone.0171359

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© 2017 Wang et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — A: phase-contrast image of NSCs globes cultured 5d in NSCs culture medium. B: Immunostaining of NSCs with Nestin antibody. C-F: immunostaining of differentiated cells with astrocyte marker GFAP, neuron marker Tuj-1 and oligodendrocyte marker O4 in 10% FBS-DF₁₂ for 5 days. Scale bar: A-B 400 um; C-F 20 um.