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. 2017 Feb 24;12(2):e0171386. doi: 10.1371/journal.pone.0171386

Fig 5. Neutrophil capture on with EC or PC monolayers or EC/PC bilayers.

Fig 5

(a) Quantification of neutrophil capture within EC monolayers, PC monolayers, or EC/PC bilayers on transwells (TWs), 10kDa hydrogels, or 20kDa hydrogels under quiescent or TNFα-activated conditions. *p<0.05, **p<0.01, ***p<0.001, and ****p<0.0001 when compared to TWs under the same culture conditions. Unless noted, changes between scaffolds are not significant. (b) Confocal microscopy results of neutrophils interacting with cellular layers on 20kDa hydrogel scaffolds. i and ii. Projection (i; * indicates three distinct neutrophils) and profile (ii; *neutrophils, dashed line outlines EC nuclei layer) of an EC monolayer with neutrophils. iii. Profile of PCs (dashed line outlines PC nucleus) and neutrophils (*denotes neutrophils). iv. EC/PC bilayer (apical layer: EC; basal layer: PC; dashed line outlines cell nuclei) and neutrophils (*denotes neutrophils). Scale bars are 10 microns.