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. 2017 Feb 24;12(2):e0172257. doi: 10.1371/journal.pone.0172257

Fig 2. Deletion of the SMC_prok_B domain or the Herpes_BLLF1 domain of FILIP do not interfere with its binding to non-muscle myosin IIb.

Fig 2

The indicated vectors were transfected into COS-7 cells. Immunoprecipitation was performed using an anti-FLAG antibody, and blots were probed with an anti-NMHCIIb antibody. Non-muscle myosin IIb was co-immunoprecipitated with FLAG-tagged full-length FILIP, FILIP d248-442, FILIP d248-685, FILIP d934-1111, and FILIP d872-1111. Lysates, corresponding total cell lysate fractions. Control means immunoprecipitation product or cell lysate derived from pCAGGS IRES-GFP-transfected COS-7 cells.