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. 2017 Feb 24;12(2):e0172257. doi: 10.1371/journal.pone.0172257

Fig 5. The regulation of spine length by FILIP in primary neurons requires Hsc70.

Fig 5

(A) Treatment with clofibric acid resulted in shorter spines in piriform neurons. Scale bar = 2 μm. The graph shows summary data from 554 spines from 6 neurons treated with vehicle and 571 spines from 4 neurons treated with clofibric acid. *p < 0.01 (B) Expression of FILIP d872-1111 in hippocampal neurons did not result in elongated spines. Scale bar = 1 μm. The graph shows summary data from 882 spines from 5 neurons (control), 851 spines from 5 neurons (FILIP d872-1111), and 696 spines from 5 neurons (FILIP). *p < 0.01 (C) Inhibition of the function of Hsc70 reverses the effects of FILIP on spine length. Scale bar = 1 μm. The graph shows summary data from 863 spines from 6 neurons (control/vehicle), 821 spines from 6 neurons (control/VER-155008), 603 spines from 6 neurons (FILIP/vehicle), and 741 spines from 6 neurons (FILIP/VER-155008). **p < 0.01, *p < 0.05.