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. 2017 Feb 24;12(2):e0172460. doi: 10.1371/journal.pone.0172460

Table 1. Candidate reference genes and its primer sequences.

Gene Name Gene symbol Gene Accession No Primer sequences Amplicon length
Actin ACTIN XM_012232381.1 F:5'-TCTCGACTACGAGCAGGAGC-3' R:5'-CGGAATCGCTCAGCACCAAT-3' 108bp
Elongation factor 1-alpha EF XM_012226913.1 F:5'-GTCTGTTGAGATGCACCATGAAG-3' R:5'-TAGAGGCAACAAAACCACGTTTC-3' 108bp
Zinc finger BED domain-containing protein DAYSLEEPER-like SLEEPER XM_012217757.1 F:5'-CGTTGCACATAGTGGAACATCC-3' R:5'-AAATTGACACGCATTCCCCTTG-3' 103bp
Glyceraldehyde 3-phosphate dehydrogenase GAPDH1 XM_012231954.1 F:5'-TCTGCTGATGCACCAATGTTTG-3' R:5'-ACCTTAGCAAGAGGAGCAAGAC-3' 116bp
NADP-dependent-glyceraldehyde 3-phosphate dehydrogenase GAPDH2 XM_012221054.1 F:5'-GTCTGGGGATTTGGTGTCGTAT-3' R:5'-GGATAGTTGAAGGGTGGGATAGC-3' 165bp
Tubulin beta-5 chain TUB XM_012218098.1 F:5'-TTCTGGAATGGGAACTCTGTTGA-3' R:5'-TTGATGTACAGAAAGGGTGGCAT-3' 142bp
Ubiquitin-conjugating enzyme E2 32 UBI XM_012228705.1 F:5'-TCAAGTGCTAACTCTGAGTCCAC-3' R:5'-CAGCAGTATTTGACCTTGTGGTG-3' 127bp
Ubiquitin domain-containing protein DSK2a DSK2A XM_012210059.1 F:5'-AAAGGTGTCGGCTTTAGGGAAT-3' R:5'-TCCACCATGGATGACTGACAAG-3' 104bp
Peptidyl-prolyl cis-trans isomerase CYP21-4 CYC XM_012227533.1 F:5'- AGTGATAGTCTTGGCGCTGC -3' R:5'- GATCTGGGATAGGTGCAGTTGT -3' 161bp
Phospholipase A I PLA XM_012235990.1 F:5'-CGTTGAGCAAAGCTGGTTCC -3' R:5'- CACAACCATCGCCAAATCCC-3' 103bp