Table 5. Most stable and least stable reference genes based on RefFinder analysis.
| Ranking Order (Better—Good—Average) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Method | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
| Staminate | ||||||||||
| Delta CT | TUB | EF | GAPDH1 | SLEEPER | DSK2A | GAPDH2 | CYC | UBI | ACTIN | PLA |
| BestKeeper | EF | GAPDH1 | TUB | SLEEPER | DSK2A | GAPDH2 | UBI | CYC | ACTIN | PLA |
| Normfinder | TUB | EF | SLEEPER | GAPDH1 | GAPDH2 | DSK2A | CYC | UBI | ACTIN | PLA |
| Genorm | EF/UBI | TUB | GAPDH1 | SLEEPER | GAPDH2 | DSK2A | CYC | PLA | ACTIN | |
| Recommended comprehensive ranking | TUB | EF | GAPDH1 | DSK2A | SLEEPER | GAPDH2 | UBI | CYC | ACTIN | PLA |
| Pistillate | ||||||||||
| Delta CT | GAPDH1 | EF | SLEEPER | CYC | TUB | GAPDH2 | DSK2A | UBI | ACTIN | PLA |
| BestKeeper | EF | GAPDH1 | CYC | TUB | SLEEPER | DSK2A | UBI | GAPDH2 | ACTIN | PLA |
| Normfinder | GAPDH1 | SLEEPER | EF | CYC | GAPDH2 | TUB | DSK2A | UBI | ACTIN | PLA |
| Genorm | EF/UBI | CYC | GAPDH1 | SLEEPER | TUB | GAPDH2 | PLA | DSK2A | ACTIN | |
| Recommended comprehensive ranking | GAPDH1 | EF | SLEEPER | CYC | TUB | DSK2A | GAPDH2 | UBI | ACTIN | PLA |
| Total | ||||||||||
| Delta CT | EF | GAPDH1 | SLEEPER | TUB | GAPDH2 | DSK2A | CYC | UBI | ACTIN | PLA |
| BestKeeper | EF | GAPDH1 | TUB | SLEEPER | DSK2A | CYC | GAPDH2 | UBI | ACTIN | PLA |
| Normfinder | SLEEPER | EF | GAPDH1 | TUB | GAPDH2 | DSK2A | CYC | UBI | ACTIN | PLA |
| Genorm | EF/UBI | GAPDH2 | GAPDH1 | SLEEPER | TUB | CYC | DSK2A | PLA | ACTIN | |
| Recommended comprehensive ranking | EF | GAPDH1 | SLEEPER | DSK2A | TUB | GAPDH2 | UBI | CYC | ACTIN | PLA |