Fig 4. Robust functional response to WNV by NK cells from younger and older healthy subjects.

PBMCs from young (n = 20) and old (n = 14) healthy subjects were incubated with medium alone (mock) or infected with WNV (MOI = 1) for 24 h. (A) Total NK cells from mock and WNV-infected groups of each WNV subject were compared by mass cytometry for surface expression of CD107a and production of production of MIP-1β, perforin, IFN-γ, GM-CSF, and TNF. Mock-WNV comparisons, paired Wilcoxon tests. Mock-mock and WNV-WNV comparisons, Mann-Whitney U tests. (B) Four NK cell subsets from young and old healthy subjects were compared for induction level of IFN-γ by subtracting baseline of the mock group from the WNV-infected group. Mann-Whitney U tests.