Table 1. Number of rye plants inoculated with VIGS-BSMV control vectors (BSMV:PDS) and vectors designed for PTGS containing cDNA fragment of ScBx1 (BSMV:ScBx1).
Vector Name | Vector design | Number of inoculated plants | Number of plants with symptoms of PDS silencing | Inoculation efficiency [%] |
---|---|---|---|---|
BSMV:PDS | α,β(-),γ(-) | 142 | 13 | 9.1 |
α,β(-),γ(PDS) | ||||
α,β(PDS),γ(PDS) | ||||
BSMV:ScBx1 | α,β(Bx),γ(PDS) | 192 | 20 | 10.4 |
α,β(PDS),γ(Bx) | 118 | 11 | 9.3 |