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. 2017 Feb 24;12(2):e0172736. doi: 10.1371/journal.pone.0172736

Fig 2. Slit1-3 and Robo1-4 mRNA expression in adult mouse (C57BL/6) spinal cord, DRG and sciatic nerve.

Fig 2

(A) RT-PCR shows Slit1-3 and Robo1-4 mRNA expression in spinal cord (SP), DRG and sciatic nerve (SN). Slit1-3, Robo1-2 and Robo4 mRNA are expressed in spinal cord and DRG. In sciatic nerve, only Slit2, Slit3, Robo1 and Robo4 mRNA are detectable. Robo3 mRNA is not expressed in the adult mouse spinal cord and the peripheral nervous system. (B) Validation of Robo3 primers with a mouse Robo3 cDNA plasmid (Robo3). (C-H) qRT-PCR compares Slit1-3, Robo1-2 and Robo4 relative expression levels between spinal cord, DRG and sciatic nerve samples. The highest mRNA level has been set as 100% for each individual graph. Error bars show one standard deviation of the mean from 3 repeat experiments. Slit1 and Robo2 have their highest relative mRNA level in the DRG. Slit2, Slit3 and Robo4 have their highest mRNA level in the sciatic nerve. Robo1 has its highest mRNA level in spinal cord. (I-J), comparison of Slit2, Slit3, Robo1 and Robo4 relative levels from two microarray data sets (GSE30165 and GSE51650). As for Panels C-H, the highest mRNA level has been set as 100% for each individual graph. This analysis also shows that Slit2, Slit3 and Robo4 appear to have their highest mRNA level in the sciatic nerve. Robo1 appears to have its highest mRNA level in the motor neurons of the spinal cord.