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. 2017 Feb 24;12(2):e0172736. doi: 10.1371/journal.pone.0172736

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© 2017 Carr et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — Double staining of Slit1-3 and Robo1-2 with NeuN in the DRG shows all sensory neurons in the DRG express Slit1-3 and Robo1-2. The neuronal marker NeuN labels both large and small diameter sensory neurons. Merged images show that Slit1 (A), Slit2 (B), Slit3 (C), Robo1 (D) and Robo2 (E) are all expressed by both large and small diameter sensory neurons. The yellow colour in merged images shows the co-localization of Slit1-3 and Robo1-2 signal with NeuN staining. Double staining of NeuN with Slit1-3 and Robo1-2 antibodies thus confirms that all the sensory neurons in the DRG express Slit1-3 and Robo1-2. Staining with Hoechst dye (Ho) is also shown (blue) to identify cell nuclei within the tissue.