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. 2017 Feb 24;12(2):e0172736. doi: 10.1371/journal.pone.0172736

Fig 9. Astrocytes of the spinal cord express Robo1.

Fig 9

(A) Double staining for Robo1 with neurofilament heavy chain antibody shows that very few axons within the spinal cord are Robo1 positive. (B) The Robo1 signal does not co-localize with the GFP signal in the spinal cord of PLP-GFP mice, which labels oligodendrocytes with cytoplasmic GFP. (C) The Robo1 signal co-localizes with the GFP signal in the spinal cord of GFAP-GFP mice, which labels astrocytes with GFP (indicated by white arrow). The staining confirms that Robo1 positive signal from outside of the neuronal cell bodies in the grey matter is from astrocytes. Staining with Hoechst dye (Ho) is also shown (blue) to identify cell nuclei within the tissue.