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. 2017 Feb 24;12(2):e0172736. doi: 10.1371/journal.pone.0172736

Fig 13. Slit2-3 and Robo1-2 staining on the sciatic nerve transverse sections from PLP-GFP mice.

Fig 13

Lower magnification images show the staining of Slit2, Slit3, Robo1 and Robo2 on sciatic nerve transverse sections from PLP-GFP mice. The PLP-GFP mice express cytoplasmic GFP in both myelinating and non-myelinating Schwann cells. Slit2, Slit3 and Robo1 staining in the sciatic nerve transverse sections from PLP-GFP mice shows Slit2 (A), Slit3 (B) and Robo1 (C) co-localization with the GFP signal. In contrast, Robo2 (D) doesn’t show any co-localization with the GFP signal (D). Slit2, Slit3 and Robo1 are expressed by the cells in the epineurium (indicated by white arrows) but not Robo2 (D). Staining with Hoechst dye (Ho) is also shown (blue) to identify cell nuclei within the tissue.