Five days after administering bleomycin, Evans blue dye analysis (A), lung wet-to-dry-weight ratio (B), BAL fluid total protein (C), MDA (D), MMP-9 (E) and PAI-1 (F) secretion in BAL fluid, total collagen (G), and hydroxyproline (OH) of lung tissue (H) were from the lungs of nonventilated control mice and mice ventilated at a tidal volume 30 mL/kg for 5 h with room air (n = 5 per group). ANOVA was used to assess the statistical significance of the differences, followed by multiple comparisons with a Scheffeˊs test, and a P value < 0.05 was considered statistically significant. * P< 0.05 versus the nonventilated control mice with bleomycin pretreatment; †P< 0.05 versus Akt+/- group.