Table 1b. Differentially expressed mRNA important for promoting aneuploidy by altering genes important for the segregation machinery.

PROMOTION OF ANEUPLOIDY
Gene	Function	V vs PF*	P3 vs PF	V vs P3
Segregation Machinery
BRCA1	Centrosomal microtubule nucleation		−5.76
CEP41	Centrosomal protein	+2.37	+16.62
CEP128	Centrosomal protein		+7.62
CEP170B	Microtubule organization	−2.19
CKAP	Stabilizes microtubules	−2.43
KIF11	Motor protein in centrosome separation		−4.67
KIF20A	Motor protein in centrosome separation		−3.45
KIF23	Kinesin – chromosome movement in division		−3.76	+2.11
^#KIF2A	Motor protein – normal mitotic progression	−2.40	−2.41
^KIF3A	Kinesin – chromosome movement in division		+3.37
KNSTRN	Mitotic spindle component		−2.39
NDE1	Microtubule organization and mitosis	−2.22
NIN	Important for centrosomal function		−2.94
SDCCAG8	Centrosome associated protein	−3.41	−4.37
SFI1	Mitotic spindle assembly		−2.23
SPAG5	Associated with mitotic spindle		−2.61
SUV39H1	Loss causes chromosome instability	−2.11	−3.05
TACC3	Stabilizes mitotic spindles		−2.78	+2.16
TOP2A	Topoisomerase – chromatid separation	−2.67	−3.30
TOPBP1	Topoisomerase binding protein	−2.01	−2.44
TPX2	Mitotic spindle assembly factor	−2.70	−3.43
#TUBB	Major component of microtubules		−2.25
TUBB2B	Major component of microtubules		+3.26
#TUBB4B	Major component of microtubules	−2.07

Gene name, function and fold change for each gene are listed; downregulated genes indicated by negative numbers, upregulated genes indicated by positive numbers.

^*Comparisons are made between empty vector and PAX3-FOXO1 (V vs. PF), PAX3 and PAX3-FOXO1 (P3 vs. PF) or empty vector and PAX3 (V vs. P3)

^{^}indicates genes demonstrated to have PAX3-FOXO1 binding sites present in their proximal promoter [29]

^#indicates genes that demonstrate similar trends in gene expression changes in human tumor samples [30–33].