Table 1c. Differentially expressed mRNA important for regulating proliferative control by altering genes for proliferative transcription factors and cell cycle regulatory proteins.
| PROLIFERATIVE CONTROL | ||||
|---|---|---|---|---|
| Gene | Function | V vs PF* | P3 vs PF | V vs P3 |
| Transcription Factors | ||||
| bcl6 | Suppresses cellular proliferation | +5.94 | ||
| #CREB3 | Promotes cellular proliferation | +2.25 | +2.48 | |
| FOXM1 | Promotes entry into S- and M-phase | −2.45 | +1.88 | |
| ^#FOXO1 | Promotes cellular proliferation | +3.16 | +2.19 | |
| FOXO4 | Promotes cellular proliferation | +3.16 | +3.84 | |
| ^#GADD45A | Inhibits entry into S-phase | +2.22 | ||
| HOXB9 | Promotes cellular proliferation | +42.81 | +9.25 | |
| KLF5 | Promotes cellular proliferation | +2.83 | +2.06 | |
| #MYC | Promotes cellular proliferation | +23.59 | −27.09 | |
| NAB2 | Negative cofactor – inhibits proliferation | +2.60 | ||
| NDN | Prevents excessive proliferation | −2.03 | ||
| #Nupr1 | Inhibits cellular proliferation | +3.10 | ||
| PML | Promotes cellular proliferation | −4.86 | ||
| Suv39h1 | Promotes cellular proliferation | −2.11 | −3.05 | |
| #TFDP1 | Promotes E2F activity/cellular proliferation | −2.06 | ||
| UHRF1 | Regulates G1/S transition | −4.00 | ||
| WRD6 | G1 arrest – inhibits proliferation | −2.57 | ||
| Cell Cycle Regulatory Proteins | ||||
| ARID3A | Promotes E2F transcription and growth | +4.53 | +4.38 | |
| #BTG2 | Anti-proliferative – inhibits Cyclin D1 | −2.07 | ||
| CCNA2 | Cyclin A2 – G1/S and G2?M progression | −2.45 | ||
| #CCND1 | Cyclin D1 – G1 progression | −5.46 | −4.32 | |
| #CCND2 | Cyclin D2 – G1 progression | −5.54 | −4.38 | |
| #CCND3 | Cyclin D3 – G1 progression | +4.03 | ||
| CCNG1 | Cyclin G1 – negative regulator of cell cycle | −2.19 | −2.66 | |
| CCPG1 | Positive regulator of proliferation | +2.93 | ||
| CCR | Increase in G1/S – promotes proliferation | −2.57 | +2.03 | |
| CDC20 | Required for completion of mitosis | −2.27 | −2.71 | |
| CDC25B | Required for G2/M progression | +2.20 | ||
| CDK1 | Promotes G2/M progression | −3.01 | −2.71 | |
| ^#CDK6 | Promotes G1 progression | −2.81 | ||
| CDK14 | Promotes G1 progression | −4.56 | ||
| CDK2AP | Interacts with CDK2 – promotes proliferation | +2.48 | ||
| cdkn1a (p21) | Inhibitor of G1 progression | +2.19 | ||
| cdkn1c (p57) | Inhibitor of G1 progression | +21.71 | +134.36 | −5.31 |
| cdkn2d (p19) | Inhibitor of G1 progression | +7.78 | +2.46 | |
| DBF4 | Required for S-phase progression | −2.57 | ||
| ^#GAS1 | Block entry into S-phase | +4.26 | −3.63 | |
| Rb1 | Tumor suppressor – inhibits proliferation | +2.10 | −2.53 | |
| Mdm2 | Facilitates G1/S phase transition | −2.11 | ||
| NEK6 | Required for M-phase progression | −4.06 | ||
Gene name, function and fold change for each gene are listed; downregulated genes indicated by negative numbers, upregulated genes indicated by positive numbers.
*
Comparisons are made between empty vector and PAX3-FOXO1 (V vs. PF), PAX3 and PAX3-FOXO1 (P3 vs. PF) or empty vector and PAX3 (V vs. P3)
^
indicates genes demonstrated to have PAX3-FOXO1 binding sites present in their proximal promoter [29]