Table 3A. Quantification of PAX3-FOXO1-dependent aneuploidy on cells stably transduced with empty vector (Vector), PAX3, PAX3-FOXO1, or the PAX3-FOXO1 phosphomutants (S201A, S205A, and S209A).
| Sample | Chromosome Number | |||||
|---|---|---|---|---|---|---|
| Hypodiploid | Diploid | Hyperdiploid/Hypotetraploid | Tetraploid | Hypertetraploid | ||
| Number cells analyzed | <2N (<40) | 2N (40) | >2N to <4N (41 – 79) | 4N (80) | >4N (>80) | |
| Vector | 62 | 7 (11.3%) | 25 (40.3%) | 7 (11.3%) | 23 (37.1%) | 0 (0.0%) |
| PAX3 | 50 | 2 (4.0%) | 34 (68.0%) | 2 (4.0%) | 12 (24.0%) | 0 (0.0%) |
| PAX3-FOXO1 | 103 | 51 (49.5%) | 6 (5.8%) | 42 (40.8%) | 4 (3.9 %) | 0 (0.0%) |
| S201A | 64 | 29 (45.3%) | 5 (7.8%) | 27 (42.2%) | 2 (3.1%) | 1 (1.5%) |
| S205A | 69 | 13 (18.8%) | 3 (4.3%) | 45 (65.2%) | 4 (5.8%) | 4 (5.8%) |
| S209A | 57 | 18 (31.6%) | 2 (3.5%) | 29 (50.8%) | 0 (0.0%) | 8 (14.0%) |
The chromosome number for individual cells was determined and classified as hypodiploid (<2N), diploid (2N), hyperdiploid/hypotetrapoid (>2N to <4N), tetraploid (4N) or hypertetraploid (>4N)