Table 3B. Quantification of PAX3-FOXO1-dependent chromosomal structural abnormalities.
Sample | Number cells analyzed | Sister Chromatid Dissociation | Telomere Association | Double Minutes | |||
---|---|---|---|---|---|---|---|
Number of Cells | Range of Events/Cell | Number of Cells | Range of Events/Cell | Number of Cells | Range of Events/Cell | ||
Vector | 62 | 1 (1.6%) | 2 | 2 (3.2%) | 2 | 0 (0.0%) | 0 |
PAX3 | 50 | 2 (4.0%) | 2 | 2 (4.0%) | 2 | 0 (0.0%) | 0 |
PAX3-FOXO1 | 103 | 16 (15.5%) | 1 – 5 | 17 (16.5%) | 1 – 16 | 4 (3.9%) | 5 – 20 |
S201A | 64 | 6 (9.4%) | 2 – 6 | 21 (32.8%) | 2 – 18 | 4 (6.3%) | 6 – 12 |
S205A | 69 | 9 (13.0%) | 1 – 3 | 20 (28.9%) | 1 – 4 | 5 (7.2%) | 1 – 62 |
S209A | 57 | 7 (12.3%) | 5 – 63 | 6 (10.5%) | 1 – 3 | 4 (7.0%) | 5 – 40 |
Metaphase chromosome analysis on cells stably transduced with empty vector (Vector), PAX3, PAX3-FOXO1, or the PAX3-FOXO1 phosphomutants (S201A, S205A, or S209A). The number of cells containing the indicated structural abnormality along with a range of the number of occurrences of the abnormality within each cell is listed.