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. 2016 Aug 11;7(39):62836–62861. doi: 10.18632/oncotarget.11229

Figure 6. Antcin M failed to protect HG-induced oxidative stress in Nrf2 silenced cells.

Figure 6

A. HNDFs were transfected with specific siRNA against Nrf2 or control siRNA. After transfection for 24 h, cells were incubated with ANM (10 μM) in the presence of HG for 12 h. Total RNA was extracted and subjected to Q-PCR analysis to determine HO-1 and NQO-1 mRNA expression levels. B. HNDFs were transfected with specific siRNA against Nrf2 or control siRNA. After transfection for 24 h, cells were incubated with ANM (10 μM) or NAC (100 μM) or RES (5 μM) in the presence of HG for 24 h. Intracellular ROS was measured by DCFH-DA assay. C. HNDFs were transfected with specific siRNA against Nrf2 or control siRNA. After transfection for 24 h, cells were incubated with ANM (10 μM) in the presence of HG for 72 h. SA-β-gal activity was measured. Results expressed as mean ± S.E.M of three indipendent expriments. Statistical significance was set at Ф, P < 0.05 compared to NG vs. HG or ANM alone or NAC alone and *P < 0.05 compared to HG vs. samples.