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. 2016 Aug 11;7(39):62836–62861. doi: 10.18632/oncotarget.11229

Figure 8. Antcin M protects wild-type C. elegans from oxidative stress and extent life span.

Figure 8

A. To determine oxidative stress resistence, age synchronized wild-type L1 larvae were pretreated with ANM (10 and 20 μM) or DMSO (0.1%) for 3 days. Oxidative stress was induced by incubation of pre-treated worms with 250 μM Juglone for 2.5, 3.5 and 4.5 h and then scored for viability. Results expressed as mean ± S.E.M of triplicate assays. Statistical significance at *P < 0.05 compared to control vs. sample treatment. B. Effect of ANM on the life span of C. elegans under hyperglycemic condition. Age synchronized L1 larvae were cultured in NGM plates wich contain HG (50 mM) with or with out ANM (10 μM) and worm were developed to adulthood. The survival rate was scored everyday and is expressed as a percentage of survival. C. Effect of ANM (10 μM) or RES (438 μM) on the life span extention of C. elegans under normal condition. Results expressed as mean ± S.E.M of three indipendent expriments. Statistical significance at ФP < 0.05 compared to control vs HG, *P < 0.05 compared to HG vs HG+sample and ΔP < 0.05 compared to control vs samples.