Figure 5. Role of TP53 and CTCF in regulation of DLL4 gene expression.
A. Ideogram representing primers used in this ChIP assay for DLL4 proximal promoter region (upper panel) and regulation of DLL4 gene expression in LFS and MCF7 cells by ChIP assay. HS27 cells were used as control (lower panel). B. Ideogram representing primers used in this ChIP assay for DLL4 distal promoter region (upper panel) and regulation of DLL4 gene expression in LFS and MCF7 cells by ChIP assay in DLL4 proximal promoter region. HS27 cells were used as control (lower panel). C. TP53 and CTCF protein levels in LFS, MCF7 and IMR32 cancer cell lines. D. Determining the interaction between TP53 and CTCF by co-immunoprecipitation.