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. 2016 Aug 4;7(39):63106–63123. doi: 10.18632/oncotarget.11056

Figure 1. Under anoxia, radiation-induced PIKK activation is markedly inhibited by GDC-0941.

Figure 1

PIKK activation was assessed via analysis of phospho(p)PIKK protein expression. In FTC-133 and 8505c cells, GDC-0941 inhibited expression of pATR at concentrations ≤ 1 μM under both normoxia and anoxia. Under normoxia, GDC-0941 inhibited pDNA-PKcs expression at higher concentrations; ≤ 10 μM in FTC-133′s and 100 μM 8505c's. Under anoxia, pDNA-PKcs was much more sensitive to GDC-0941, with almost complete inhibition observed at 1 μM GDC-0941 in both cell lines. Under normoxia, GDC-0941 had little effect on pATM, whilst a clear inhibition was observed in both cell lines under anoxia. Cells were exposed to GDC-0941 in normoxia/anoxia for 18 h, irradiated (4 Gy) whilst remaining in the specified conditions and lysates collected 1 h later for analysis. Cropped blots are representative of 4 independent experiments.