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. 2016 Aug 4;7(39):63106–63123. doi: 10.18632/oncotarget.11056

Figure 2. Under anoxia, genetic inhibition of PI3K inhibits radiation-induced ATM and DNA-PKcs activation.

Figure 2

PIKK activation was assessed via analysis of phospho(p)PIKK protein expression. Genetic inhibition of PI3K (via PTEN reconstitution into PTEN-null FTC-133 cells) had little effect on pATR expression under normoxia and anoxia. Radiation-induced pATM and pDNA-PKcs were inhibited in anoxic PTEN-expressing cells. PCI NEO and PTEN reconstituted FTC-133 cells were incubated in normoxia/anoxia for 18 h irradiated (4 Gy) whilst remaining in the specified conditions and lysates collected 1 h later for analysis. Cropped blots are representative of 4 independent experiments.