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. 2016 Aug 12;7(39):63388–63407. doi: 10.18632/oncotarget.11255

Figure 3. HuR silencing effects on cell viability.

Figure 3

Panel A. Nthy-ori-3.1 and BCPAP cells were transfected with non-targeting siRNA (NC, negative control) or three different siRNA sequence specific to HuR (5 nM) and collected after 72 h treatment. HuR protein levels were analyzed by Western Blot analysis, as described in Materials and Methods section. Panel B-C. Nthy-ori-3.1 (B) and BCPAP (C) were transfected to either siRNA1 or siRNA2 or negative control for 72 hours and cell death phenomena was analyzed by Annexin V and PI staining. Panel D. Histogram relative to Annexin V/PI analysis.