Figure 4. ALOS4 dose-dependently increases survival of mice and reduces tumor mass in subcutaneous melanoma model.

Mice (16 week-old, C57BL/6; n=10 each group) were inoculated s.c. with B16F10 cells (5.0 × 10⁴ cells/ml) and injected daily with ALOS4 (0.1, 0.3 or 0.5 mg/kg, i.p.) from day 1-60 post-inoculation. Results were similar for all ALOS4 doses examined; only 0.3 mg/kg ALOS4 shown for clarity. A. ALOS4-treated mice had significantly higher survival (Mantel-Cox, P<0.0001; Log-rank test for trend, p=0.0016). Median survival date of animals: Saline control, 36.5 days; ALOS4 0.3 mg/kg-treated, could not be estimated. B. Palpable tumor mass was estimated by (π/6 × width × length × height). Bars represent mean tumor volume (±SEM). Asterisks represent results of a Bonferroni means separation test between doses (two-way ANOVA, p=0.0002; *, p<0.05; ***, p<0.001).