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. 2016 Aug 23;7(39):63629–63639. doi: 10.18632/oncotarget.11556

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Copyright: © 2016 Eberhardt et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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(A) Colocalization of MYCN and PRMT1 as shown by confocal images of immunostaining of PRMT1 and MYCN in LAN-5 cells. (B) Coomassie blue staining of bacterially expressed GST-MYCN (left). A full western blot of GST and GST-MYCN as detected by anti-MYCN antibody (right). (C) Coomassie blue staining of recombinant PRMT1 protein expressed from Sf9 insect cells. (D) MYCN directly binds to PRMT1 in vitro. GST pull-down assays were performed using Flag-PRMT1 with GST or GST-MYCN followed by immunoblotting with anti-Flag antibody. (E) Endogenous MYCN physically interacts with PRMT1. Nuclear extracts from LAN-5 cells were subjected to CoIP analysis with α-MYCN antibody, followed by immunoblotting with α-PRMT1 antibody.