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. 2016 Aug 22;7(39):63651–63660. doi: 10.18632/oncotarget.11467

Figure 1. TLR2-mediated induction mRNA expression of IL-23p19 in PC-like cells.

Figure 1

PC-like cells were stimulated with10 μg/ml PGN, 1 μg/ml Pam3CSK4, 10 μg/ml Poly (I:C), 10 μg/ml LPS, 1 μg/ml Flagellin, 1 μg/ml FSL-1, 1 μg/ml Imiquimod, 1 μg/ml ssRNA40 and 1μM ODN2006 for 4h, 8h and 16h, then total RNA was isolated and IL-23p19 mRNA expression was determined by real-time PCR. Data are normalized to 18 S rRNA and expressed in arbitrary units (AU), representing mRNA induction compared to unstimulated cells. Data are shown as means ± SD of three independent experiments. *P < 0.05 vs. Medium group.