Figure 2. Effects of various histone deacetylase inhibitors on cell proliferation, apoptosis activation and histone modifications.

PEER cells were exposed to drugs for 48 hrs and analyzed for proliferation using the MTT assay a. Activation of apoptosis was determined using the Annexin V (Ann V; flow cytometry dot plots showing apoptosis profile for each treatment are on top of the bar graphs) assay (a) and Western blotting b. Modifications of histone 3 (b) and changes in the level of different histone deacetylases c. were determined by Western blotting. Quantitative analysis of the bands was done by normalizing the signals to β-ACTIN and calculating the ratio relative to the control (left side of panel c). d. Cells were exposed to HDAC Class II selective inhibitors LMK-235 or Nexturastat A (NA) at the indicated concentrations for 48 hrs and analyzed by Western blotting. Rom, romidepsin; Bel, belinostat, Pano, panobinostat; SAHA, suberoylanilide hydroxamic acid.