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. 2016 Aug 24;7(39):63870–63886. doi: 10.18632/oncotarget.11563

Figure 4. ROS generation induced by curcumin in combination with cisplatin in 253J-Bv and T24 bladder cancer cells.

Figure 4

A. 253J-Bv and T24 cells were incubated with 10 μM cisplatin and 10 μM curcumin for 24 h and then stained with 10 μM H2DCFDA to assess intracellular ROS levels by flow cytometry. Data are normalized to the mean fluorescence intensity of DCF in 253J-Bv and T24 cells stimulated without curcumin and cisplatin, taken as 100%. Data are expressed as the mean ± SEM of three independent experiments. Significant differences from the results obtained with cells incubated with only medium are shown. *p <0.005 compared with medium alone was considered statistically significant. B, C. 253J-Bv and T24 cells were incubated with 10 μM cisplatin and 10 μM curcumin for 24 h. The intracellular ROS generation in bladder cancer cells was detected by inverted fluorescence microscopy (400× magnification). The images represent three experiments showing similar results.