Figure 5. Activation of p-MEK and p-ERK1/2 in 253J-Bv and T24 bladder cancer cells stimulated with curcumin and cisplatin.

A, B. 253J-Bv and T24 cells were incubated for 24 h at 37°C with or without 10 μM curcumin and 10 μM cisplatin. After incubation, samples were subjected to SDS-PAGE and immunoblotted with anti-phospho-MEK or anti-MEK antibodies. The images represent three experiments showing similar results. C, D. 253J-Bv and T24 cells were incubated for 3-24 h with or without 10 μM curcumin and 10 μM cisplatin and immunoblotted with anti-phospho-ERK1/2 or anti-ERK1/2 antibodies. The images represent three experiments showing similar results.