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. 2016 Aug 30;7(39):63887–63900. doi: 10.18632/oncotarget.11698

Figure 2. FAM122A interacts with PP2A complex.

Figure 2

(A–H) 293T cells were transiently cotransfected with indicated plasmids followed by IP with anti-GFP antibody (A–D) or anti-Flag antibody (E–H). The indicated proteins were examined by western blot. (I) 293T cells were transiently transfected with GFP-tagged FAM122A or GFP expressing plasmids and IP was performed by anti-GFP antibody or non-specific IgG. Subunits of PP2A complex were detected by western blot. The symbol * indicates heavy chains of immunoglobulin. (J) The recombinant GST-FAM122A was blotted with the anti-FAM122A antibody. (K) 293T cells were stably transfected with Flag-FAM122A and empty plasmids, or with two independent shRNAs targeting FAM122A and non-specific shRNA as a negative control (NC). Western blot was applied to examine the specificity of anti-FAM122A antibody. The symbol * indicates nonspecific bands. (L, M) 293T (L) and Jurkat (M) cells were immunoprecipitated with anti-FAM122A antibody or non-specific IgG followed by western blot with indicated antibodies. 10% cell lysates (input) was used as a positive control.