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. 2016 Aug 31;7(39):64007–64020. doi: 10.18632/oncotarget.11746

Figure 1. Haploid loss of Gata3 enhances B cell populations in the bone marrow and spleen, but reduces T cell populations in the thymus.

Figure 1

(A) Tissues from the spleen or thymocytes of WT and Gata3+/− mice at 2 months of age were analyzed for Gata3 expression by Western blot (left panel) and flow cytometry (middle panel). Sorted B220+ B cells from the BM or spleen were analyzed for Gata3 expression by Q-RT-PCR (right panel). Data represent the mean ± SD from triplicates of 2 mice per genotype. (B–D). Cells from the spleen, BM and thymus of WT (n = 5) and Gata3+/− (n = 5) mice at 2 months of age were analyzed by flow cytometry. Representative profiles are shown. no., number. (E) mRNA levels of the indicated genes in thymocytes from WT and Gata3+/− mice at 2 months of age were determined by Q-RT-PCR. Data represent the mean ± SD from triplicates of 3 mice per genotype.