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. 2016 Aug 31;7(39):64007–64020. doi: 10.18632/oncotarget.11746

Figure 3. Loss of p18 partially restores Gata3 deficient thymocyte proliferation, and haploid loss of Gata3 in a p18 null background promotes B cell proliferation and differentiation.

Figure 3

(A). Splenic tissue, thymocytes, or sorted BM B cells (B220+IgM−and B220+IgM+) from p18−/− and p18−/−;Gata3+/− mice at 2 months of age were analyzed for Gata3 expression by Western blot (left panel) and flow cytometry (middle panel). Sorted B220+ B cells from the spleen and BM were analyzed for Gata3 expression by Q-RT-PCR (right panel). Data represent the mean ± SD from triplicates of 2 mice per genotype. (B, D) Cells from the BM (B) and spleen (D) of p18−/− and p18−/−;Gata3+/− mice at 2 months of age were analyzed. Results represent the mean ± SD of 5 animals per group. (C, E) BrdU incorporation in B220+ BM cells (C) and thymocytes (E) from p18−/− and p18−/−;Gata3+/− mice at 2 months of age were analyzed by flow cytometry. Results represent the mean ± SD of 3 animals per group. (F) Immunofluorescence staining of Ki67 (green, nuclear staining) and B220 (red, membrane staining) in the spleen from p18−/− and p18−/−;Gata3+/− mice. The percentagest of Ki67+ cells were calculated from B220+ cells and quantitated in five randomly selected fields in splenic sections of WT and Gata3+/− mice, and the results represent the mean ± SD of three animals per group. (G) mRNA levels of the indicated genes in thymocytes from p18−/− and p18−/−;Gata3+/− mice at 2 months of age were determined by Q-RT-PCR. Data represent the mean ± SD from triplicates of 3 mice per genotype.