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. 2016 Aug 31;7(39):64030–64042. doi: 10.18632/oncotarget.11747

Figure 1. ΔF508 mutation leads to intestinal inflammation in mice.

Figure 1

(A) Immunofluorescence staining of CFTR (Anti-CFTR, 1:20, ACL-006, Alomone Labs.) in WT and ΔF508cftr−/− mouse small intestine, scale bar = 10 μM, (B) Immunohistochemistry staining of macrophages and neutrophils (Rat anti-mouse F4/80, 1:50, Bio-Rad, Rat anti-mouse Ly-6B.2 Alloantigen, 1:50, Bio-Rad) in WT and ΔF508cftr−/− small intestine. Scale bar: 100 μm. (C) H&E staining shows significant increase of the thickness of smooth muscle layer and the depth of intestinal crypts in ΔF508cftr−/− mouse small intestine. Scale bar: 100 μm. (D) Western blot analysis compares the expression of mature CFTR protein and α-smooth muscle actin (α-SMA) in WT (n = 7) and ΔF508cftr−/− (n = 6) small intestine.