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Cells were transfected with siRNAs targeting p97 or control siRNAs (0.5 μM siRNA to 2.5 × 105 cells) and treated with 100 ng/ml TRAIL for 24 hours. Dead cells were evaluated microscopically (original magnification ×100), by flow cytometry using annexin V/propidium iodide (PI) staining (A, B), and by a caspase-3 activity assay (C, D). ** = p < 0.01.
