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. 2016 Sep 7;7(39):64221–64232. doi: 10.18632/oncotarget.11890

Figure 5. Effects of p97 inhibition in an in vivo arthritis model.

Figure 5

CIA was induced in 7-week-old Lewis rats. Scrambled or p97 siRNA (10 μM)-atelocollagen complexes were injected into ankle joints of rats 14 days after the first immunization with type II collagen (day 15). The siRNA-mediated knockdown of p97 in collagen-induced arthritis (CIA) rat synovial tissues obtained from knee and ankle joints three days after the injection of siRNA into ankle joints was verified by Western blotting (A). Serum levels of antibodies against type II collagen on the day of sacrifice (day 29) (B). Representative pictures of the ankle joints on day 22 (C). Arthritis score following the induction of CIA (D). Bone erosion was assessed by micro-CT on day 29. Cor., coronal; Sag., sagittal. (E, F). Hematoxylin and Eosin staining of the ankle joints (G). Cartilage destruction was quantified histologically (H). Hsp47 was labelled to evaluate the proliferation of fibroblasts in synovial tissues. Original magnification ×100. Values are the mean ± SD (I, J). * = p < 0.05; ** = p < 0.01.