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. 2017 Feb 24;12(2):e0172832. doi: 10.1371/journal.pone.0172832

Fig 7. OTS167 reduces transcript and protein levels of mutant p53.

Fig 7

(A) qPCR reveals that mutant p53 transcript is significantly downregulated in MDA-MB-231, BT-549 and SUM-159 after 48h and 72h (45 nM) OTS167 treatment. (B) Western blots show that mutant p53 protein is significantly reduced in MDA-MB-231, BT-549 and SUM-159, but WT p53 is increased in MCF-7 (48h, 45 nM OTS167, densitometric data from three experiments). (C) p53 protein reduction is independent of cell density (MDA-MB-231, 96h, 45nM OTS167). Significance was calculated using a Student’s two-tailed t-test: p < 0.05 (*), p < 0.01 (**), p < 0.001 (***).