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. 2017 Feb 24;12(2):e0172985. doi: 10.1371/journal.pone.0172985

Fig 2. DYRK1A affected NFATc2 protein ubiquitination and stability.

Fig 2

A and B. HEK293 cells transfected with p-NFATc1mycFLAG were treated with proteasome inhibitorlactacystin (lac) for time or dosage indicated. C and D. HEK293 cells transfected with p-NFATc1mycFLAG were treated with lysosome inhibitor chloroquine (chl) for time or dosage indicated. E. Co-IP assay showed the cross-interaction between NFATc1 and ubiquitin. F and G. NFATc1 and NFATc2 were increased by inhibition of SCF E3 ligase using Cullin-1/2 DN constructs. Values represent means±SD, n = 3.Lacmeanslactacystin. Ubi means ubiqutin.