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. 2017 Feb 24;12(2):e0172812. doi: 10.1371/journal.pone.0172812

Fig 1. Histopathologic analyses of intestine and trachea tissues from SPF chickens in-ovo vaccinated with NDV strains.

Fig 1

SPF chicken embryos were inoculated with NDV strain TS09-C (103.0 EID50/egg), strain V4 (102.0 EID50/egg), or PBS via the amniotic route at 18 embryo-days. The intestine and trachea tissues were collected from immunized chickens at 5 days post-hatch, fixed in 4% paraformaldehyde, paraffin embedded, sectioned and stained with hematoxylin-eosin (H&E), and analyzed by a microscope. Scale bar = 500μm.