Fig 4. OXTR ligand binding in the adrenal gland of a P0 male mouse.

Pseudo-color composite (OXTR in red, cresyl violet counterstain in gray) (A), followed by cresyl violet counterstain (B), with black arrows indicating region of interest. OXTR ligand binding is indicated by white arrows in the 0 nM OXT competition (C), followed by 1000 nM OXT competing with OVTA radioligand to reduce the signal (D). Specific binding can be observed on the kidney (C) just below the adrenal gland, and this is also competed away by 1000 nM OXT (D). Nonspecific binding to lipids in the duodenum can be seen above the adrenal gland; this faint signal does not change across competition conditions. OXTR ligand binding in the adrenal gland (or kidney) was not present in OXTR KO mice (E), followed by the post-processed cresyl violet tissue of the OXTR KO (F).