Fig 3. Immunopathology caused by CD8 T cells is dependent on the NLRP3 inflammasome.
WT, caspase-1/11-/- or NLRP3-/- C57BL/6 mice were infected with L. major in the ear, and 2 weeks later mice were co-infected with 2×105 PFU of LCMV Armstrong strain by i.p. injection; (a and d) ear thickness was assessed weekly. Five weeks post infection with L. major, mice were euthanized and the lesions were digested and used for flow cytometric analysis of intracellular IFN-γ and GzmB on (b and e) CD4 T cells or CD8 T cells. (c and f) Number of parasites in the skin was determined at 5 weeks post infection with L. major. Graphs are data from 2 to 3 independent experiments (n = 5 mice per group) with similar results. **p<0.01