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. 2017 Feb 13;13(2):e1006196. doi: 10.1371/journal.ppat.1006196

Fig 5. IL-1β is highly expressed in human lesions and blockade of NLRP3 prevents IL-1β release from human skin biopsies.

Fig 5

Cells isolated from lesions or PBMC obtained from L. braziliensis patients were stained for flow cytometry directly ex vivo and depicted are (a) IL-1β+ CD11b+ cells (b) IL-1β expression within CD68+ (macrophages and monocytes) or CD66b+ (granulocytes) in the skin. Data obtained from (a) 19 PBMC or 18 skin lesions or (b) 13 skin lesions. (c) Punch biopsies from normal skin or L. braziliensis lesions were cultured for 48h and IL-1β was measured in the supernatants by ELISA. Data obtained from 5 normal skin samples and 14 lesions. L. braziliensis patients’ skin biopsies were cut in half and one half was cultured in media+DMSO and the other half with media+glyburide; 48 hours later, the presence of (d) IL-1β, (e) IL-6 and (f) IL-10 in the supernatants was determined by ELISA. Data obtained from 9 skin samples from two independent experiments. PBMC, peripheral blood mononuclear cells. **p<0.01 or ***p<0.001