Table 3. Inhibitory kinetics of EgKU-3 on bovine chymotrypsin A.
| Kinetic constant | Chymotrypsin |
|---|---|
| k2a | (1.2 ± 0.2) x 10−2 s-1 |
| KIa | 3.2 ± 1.2 nM |
| k2/KIa | (3.8 ± 2.0) x 106 M-1 s-1 |
| k-2b | (2.8 ± 2.3) x 10−4 s-1 |
| KI*c | 73 ± 30 pM |
ak2, KI and k2/KI were calculated from time course experiments (see Fig 2B and 2C) according to the fit to Eq 5 of kobs versus [I] plots. Values are averages of independent measurements ± the standard error (n ≥ 2).
bk-2 was calculated from time course experiments according to Eq 6. The value is the average of independent measurements ± the standard deviation (n = 15).
cKI* was calculated from Eq 7 using the values of k2, KI and k-2 obtained from time course experiments. The value is the average of independent measurements ± the standard error (n ≥ 3).