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. Author manuscript; available in PMC: 2018 Feb 8.
Published in final edited form as: Cell Host Microbe. 2017 Feb 8;21(2):182–194. doi: 10.1016/j.chom.2017.01.009

Figure 5. CCR7-dependent cell migration is necessary for hyperdissemination by ST313 S. Typhimurium.

Figure 5

(A–I) Streptomycin-pretreated WT C57BL/6 or Ccr7−/− mice were orally infected with each Salmonella isolate. Mice were sacrificed at 2 dpi and Salmonella burden was enumerated by plating. The CFU/g for systemic sites (A–C) are presented. CFU/organ for the MLN is presented in Figure S5. (D–I) Single cell suspensions of the MLN were stained for cell surface markers and intracellular Salmonella. The total number of infected cells per MLN (D, G), percent infected (E, H) and total number of cells recruited to the MLN (F, I) are shown for all migDC (D–F) and the CD11b+ migDC subset (G–I), respectively. (A–I) Data are combined from 2 independent experiments with 4–5 mice per group. The geometric mean (A–C) or mean (D–I) for each group is shown and statistical significance was determined by the Mann-Whitney test. * P≤0.05, ** P≤0.01, *** P≤0.001, **** P≤0.0001. See also Figure S5.