Figure 6. Pseudogenization of SseI in ST313 S. Typhimurium contributes to hyperdissemination in CD11b⁺ DCs.

(A–G) Streptomycin-pretreated C57BL/6 mice were orally infected with each respective Salmonella strain. To genetically dissect the effect of pseudogenization of sseI, a clean deletion of sseI was made in ST19 background and D23580 was complemented with a functional copy of SseI. Mice were sacrificed 2 dpi, and Salmonella burden was enumerated by plating. CFU/g of each strain in the MLN is shown in A. The CFU/organ in the MLN is presented in Figure S6. (B–G) Single cell suspensions of the MLN were stained for cell surface markers and intracellular Salmonella. The total amount of infected cells per MLN (B, E), percent infected (C, F) and total number of cells recruited to the MLN (D, G) are shown for all migDC (B–D) and the CD11b+ migDC subset (E–G), respectively. (A–G) Data presented are the combination of 2 independent experiments with 4–5 mice per group. The geometric mean for each group is shown and statistical significance was determined by the Mann-Whitney test.

*P≤0.05, ** P≤0.01, *** P≤0.001. See also Figure S6.