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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Virology. 2017 Jan 23;503:52–61. doi: 10.1016/j.virol.2017.01.009

Fig 2.

Fig 2

Comparison of HBV RNA and envelope protein levels in Huh7 cells transfected with dimeric constructs of genotype A and genotype D. SphI dimers of 6 genotype A clones and 7 genotype D clones were transiently transfected to Huh7 cells, followed by Northern blot analysis of HBV RNAs (A) as well as Western blot analysis of intracellular and secreted envelope proteins (B and C). For Northern blot, positions of 3.5-kb, 2.4/2.1-kb and 0.7-kb HBV RNAs are indicated, and the same blot was subsequently hybridized with a GAPDH probe for loading control. For cell lysate, the same blot was sequentially probed with polyclonal anti-S antibody from Novus, Abcam, and anti-actin antibody for loading control. Positions of L, M, and S proteins are indicated. Western blot analysis of viral envelope proteins from culture supernatant was preceded by PEG precipitation.