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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Biochim Biophys Acta. 2017 Jan 14;1860(3):374–382. doi: 10.1016/j.bbagrm.2017.01.005

Fig. 4. Fhit mediates translational regulation through the TK1 5’-UTR.

Fig. 4

A. H1299 D1 and D1 induced cells were transfected with a plasmid expressing firefly luciferase with no added 5’-UTR (control, grey bars), or the same plasmid expressing firefly luciferase with the TK1 5’-UTR (TK1, black bars). Results were normalized to co-transfected Renilla luciferase. Fhit induction is shown in the Western blot at the top. B. The experiment in A was repeated using Fhit-negative H1299 E1 cells treated with or without ponasterone A. Data shown in A and B are the means ±SEM of three independent experiments (each measured in triplicate). The asterisk (*) indicates p <0.05 as determined by two-tailed Student T test.